Human CCL20/MIP-3 alpha ELISA Kit
Cat no : KE00149
Synonyms
Beta chemokine exodus 1, C C motif chemokine 20, CC chemokine LARC, CCL20, CKb4, LARC, MIP 3 alpha, MIP 3a, MIP3A, SCYA20, Small inducible cytokine A20, ST38
Validation Data Gallery
Product Information
KE00149 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL20 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL20. The assay recognizes human CCL20. An antibody specific for CCL20 has been pre-coated onto the microwells. The CCL20 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL20 is added to detect the captured human CCL20 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCL20/MIP-3 alpha ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.9 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6364 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 74%-127% |
| Cell culture supernatants | 94% | 70%-129% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 171.7 | 5.6 | 3.3 |
| 2 | 20 | 42.6 | 0.8 | 1.8 |
| 3 | 20 | 9.8 | 0.3 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 176.0 | 6.0 | 3.4 |
| 2 | 24 | 42.2 | 0.8 | 1.8 |
| 3 | 24 | 10.4 | 0.4 | 3.6 |
Background Information
CCL20, also known as MIP-3 alpha, is a chemokine expressed mainly in the lymphatic tissue, lung and liver produced by cells related with inflammation and autoimmune response such as endothelial cells, neutrophils, natural killer (NK), Th17 cells and B cells among others. CCL20 contributes to inflammatory cell recruitment. CCL20 selectively signals through its receptor CCR6. The ligand-receptor pair CCL20/CCR6 is responsible for the chemoattraction of immature dendritic cells, effector/memory T cells and B cells, and also serves a role in the skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathological processes, such as those associated with cancer and rheumatoid arthritis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Beta chemokine exodus 1, C C motif chemokine 20, CC chemokine LARC, CCL20, CKb4, LARC, MIP 3 alpha, MIP 3a, MIP3A, SCYA20, Small inducible cytokine A20, ST38 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Stem Cell Res Ther CCL20/CCR6 axis mediates macrophages to promote proliferation and migration of ESCs by blocking autophagic flux in endometriosis | ||
Front Oncol A Novel Secreted Protein-Related Gene Signature Predicts Overall Survival and Is Associated With Tumor Immunity in Patients With Lung Adenocarcinoma. | ||
Cell Death Discov ADO/hypotaurine: a novel metabolic pathway contributing to glioblastoma development. | ||
Front Cell Dev Biol Enhanced T-cell activation and chemokine-associated function in CD14-positive cells from venous sinus blood in sub-acute cerebral venous sinus thrombosis | ||
Adv Sci (Weinh) CCL20/CXCL5 Drives Crosstalk Between Anaplastic Thyroid Cancer Stem Cells and Tumor-Associated Macrophages to Promote Tumor Progression | ||
Front Immunol Macrophage-derived CCL20 promotes abdominal aortic aneurysm progression via lymphocytes CCR6. |