Human CCL22/MDC ELISA Kit
Cat no : KE00189
Synonyms
A 152E5.1, C C motif chemokine 22, CC chemokine STCP 1, CCL22, DC/B CK, Macrophage derived chemokine, MDC, MDC(1 69), SCYA22, Small inducible cytokine A22, STCP 1
Validation Data Gallery
Product Information
KE00189 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL22/MDC ELISA kit is to be used to detect and quantify protein levels of endogenous CCL22/MDC. The assay recognizes human CCL22/MDC. An antibody specific for CCL22/MDC has been pre-coated onto the microwells. The CCL22/MDC protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL22/MDC is added to detect the captured human CCL22/MDC protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCL22/MDC ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6367 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 78%-113% |
| Cell culture supernatants | 98% | 85%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 230.0 | 4.1 | 1.8 |
| 2 | 20 | 55.3 | 1.1 | 2.1 |
| 3 | 20 | 12.9 | 0.4 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 239.2 | 6.9 | 2.9 |
| 2 | 24 | 54.9 | 2.0 | 3.7 |
| 3 | 24 | 13.2 | 0.7 | 5.0 |
Background Information
The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation. Accumulating studies indicate that CCL22 recruits regulatory T (T-reg) cells into tumor tissues and is expressed in many human tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | A 152E5.1, C C motif chemokine 22, CC chemokine STCP 1, CCL22, DC/B CK, Macrophage derived chemokine, MDC, MDC(1 69), SCYA22, Small inducible cytokine A22, STCP 1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Sci Rep Pyromeconic acid-enriched Erigeron annuus water extract as a cosmetic ingredient for itch relief and anti-inflammatory activity |