Speedy™ Human CCL22/MDC One-Step ELISA Kit
Cat no : SE50033
Synonyms
CCL22, MDC, C C motif chemokine 22, CC chemokine STCP-1, C-C motif chemokine 22
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CCL22/MDC One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 2.7 pg/mL |
| Range | 25-1600 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6367 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 111% | 101%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 901.0 | 36.4 | 4.0 |
| 2 | 8 | 208.5 | 15.0 | 7.2 |
| 3 | 8 | 97.6 | 1.7 | 1.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 916.9 | 32.0 | 3.5 |
| 2 | 16 | 206.0 | 13.1 | 6.4 |
| 3 | 16 | 97.3 | 2.4 | 2.5 |
Background Information
The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation. Accumulating studies indicate that CCL22 recruits regulatory T (T-reg) cells into tumor tissues and is expressed in many human tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCL22, MDC, C C motif chemokine 22, CC chemokine STCP-1, C-C motif chemokine 22 |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.