Human CCL24/Eotaxin-2/MPIF-2 ELISA Kit
Cat no : KE00196
Synonyms
C C motif chemokine 24, CCL24, CK beta 6, Ckb 6, Eotaxin 2, MPIF 2, MPIF2, SCYA24, Small inducible cytokine A24
Validation Data Gallery
Product Information
KE00196 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL24 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL24. The assay recognizes human CCL24. An antibody specific for CCL24 has been pre-coated onto the microwells. The CCL24 protein in samples is captured by the coated antibody after incubation.Following extensive washing, another antibody of biotinylated specific for CCL24 is added to detect the captured CCL24 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCL24/Eotaxin-2/MPIF-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.8 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6369 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 79%-117% |
| Cell culture supernatants | 91% | 73%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 56.6 | 4.4 | 7.8 |
| 2 | 20 | 85.9 | 5.7 | 6.6 |
| 3 | 20 | 259.5 | 12.1 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 58.2 | 2.8 | 4.9 |
| 2 | 24 | 89.1 | 6.6 | 7.4 |
| 3 | 24 | 247.7 | 16.2 | 6.5 |
Background Information
CCL24, known as eotaxin-2, is a chemokine that promotes cell trafficking and regulates inflammatory and fibrotic activities mainly through the C-C motif chemokine receptor 3 (CCR3) complex. It is expressed by activated T cells, monocytes, epithelial and endothelial cells as well as by activated fibroblasts. CCL24 is considered a contributor to kinds of atopic disorders, parasitic infections and numerous systemic diseases. CCL24 is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C C motif chemokine 24, CCL24, CK beta 6, Ckb 6, Eotaxin 2, MPIF 2, MPIF2, SCYA24, Small inducible cytokine A24 |