Human CCR2a ELISA Kit
Cat no : KE00846
Synonyms
CCR2, C C chemokine receptor type 2, C C CKR 2, C-C chemokine receptor type 2, CC CKR 2
Validation Data Gallery
Product Information
KE00846 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CCR2a ELISA kit is to be used to detect and quantify protein levels of endogenous human CCR2a. The assay recognizes human CCR2a. An antibody specific for human CCR2a has been pre-coated onto the microwells. The human CCR2a protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CCR2a is added to detect the captured human CCR2a protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCR2a ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 3.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 729230 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 97% | 86%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 539.9 | 17.5 | 3.2 |
| 2 | 8 | 117.9 | 3.0 | 2.6 |
| 3 | 8 | 51.6 | 2.6 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 519.1 | 30.0 | 5.8 |
| 2 | 16 | 116.1 | 4.3 | 3.7 |
| 3 | 16 | 51.2 | 3.1 | 6.1 |
Background Information
CCR2 (C-C motif chemokine receptor-2) is a transmembrane protein highly expressed in bone marrow and lymph nodes. It belongs to the G-protein-coupled seven-transmembrane receptor superfamily. CCR2 is the key functional receptor for MCP-1 (Monocyte chemoattractant protein-1). CCR2 and monocyte chemoattractant proteins (MCPs) play pivotal roles in the development of inflammatory responses and are crucial for the recruitment of immune cells to sites of inflammation. There are two isoforms of CCR2, CCR2a and CCR2b. CCR2a was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCR2, C C chemokine receptor type 2, C C CKR 2, C-C chemokine receptor type 2, CC CKR 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.