Human CCR5 ELISA Kit
Cat no : KE00535
Synonyms
CCR5, C C chemokine receptor type 5, C C CKR 5, C-C chemokine receptor type 5, CC CKR 5
Validation Data Gallery
Product Information
KE00535 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CCR5 ELISA kit is to be used to detect and quantify protein levels of endogenous human CCR5. The assay recognizes human CCR5. An antibody specific for human CCR5 has been pre-coated onto the microwells. The human CCR5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CCR5 is added to detect the captured human CCR5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCR5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1234 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 97% | 86%-114% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.07 | 0.51 | 5.07 |
| 2 | 8 | 2.53 | 0.15 | 5.84 |
| 3 | 8 | 1.54 | 0.05 | 3.14 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.23 | 0.51 | 4.97 |
| 2 | 16 | 2.62 | 0.16 | 6.11 |
| 3 | 16 | 1.45 | 0.12 | 8.54 |
Background Information
CCR5 (CC chemokine receptor 5) is a seven-transmembrane, G protein-coupled receptor (GPCR) that regulates trafficking and effector functions of memory/effector T-lymphocytes, macrophages, and immature dendritic cells. It also serves as the main coreceptor for the entry of R5 strains of human immunodeficiency virus (HIV-1, HIV-2). CCR5 plays a pivotal role when expressed in breast cancers in facilitating tumor progression and metastasis in multiple ways. CCR5 is also among the most studied chemokine receptors due to its profound significance in human health and disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCR5, C C chemokine receptor type 5, C C CKR 5, C-C chemokine receptor type 5, CC CKR 5 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.