Human CCT5 ELISA Kit
Cat no : KE00546
Synonyms
CCT5,CCT epsilon,CCTE,CCT-epsilon,Chaperonin conta
Validation Data Gallery
Product Information
KE00546 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CCT5 ELISA kit is to be used to detect and quantify protein levels of endogenous human CCT5. The assay recognizes human CCT5. An antibody specific for human CCT5 has been pre-coated onto the microwells. The human CCT5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CCT5 is added to detect the captured human CCT5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCT5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22948 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 96% | 90%-106% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.10 | 0.30 | 5.85 |
| 2 | 8 | 1.23 | 0.08 | 6.12 |
| 3 | 8 | 0.59 | 0.03 | 4.33 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.03 | 0.25 | 5.00 |
| 2 | 16 | 1.21 | 0.07 | 5.77 |
| 3 | 16 | 0.61 | 0.04 | 5.90 |
Background Information
CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC). CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin. The CCT5 structure harbors key functional domains, including a nucleotide-binding site, substrate-binding site, sensor loop, and apical loop, all of which are implicated in protein folding stability, a process linked to cancer pathogenesisThe expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCT5,CCT epsilon,CCTE,CCT-epsilon,Chaperonin conta |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.