Human CD14 Magnetic Beads Kit
Host / Isotype
Mouse / Mouse IgG2b, kappa
Cat no : KMS005
CD14 is a surface antigen expressed on monocyte/macrophage. It cooperates with other proteins to mediate innate immune response to bacterial LPS. 5%-10% human peripheral blood mononuclear cells (PBMC) express CD14. Human CD14 Magnetic Beads Kit is used for isolation or depletion of human CD14 monocytes/macrophages from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD14 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD14+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD14- cells remain in supernatant and can also be used for further applications.
· MS001-10: 100µL 10mg/mL streptavidin magnetic beads
· MS65246-10: 100µL 0.1mg/mL Biotin-CD14 (clone: 26IC)
· MS001-100: 1mL 10mg/mL streptavidin magnetic beads
· MS65246-100: 1mL 0.1mg/mL Biotin-CD14 (clone: 26IC)
PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide
10µL Biotin-CD14 antibody and 10µL streptavidin beads for 1*107 cells.
Cited in Article as
KMS005, Human CD14 Magnetic Beads Kit, Proteintech, IL, USA