Human CD14 Magnetic Beads Kit

Host / Isotype

Mouse / Mouse IgG2b, kappa

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

CloneNo.

26IC

Cat no : KMS005

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Validation Data Gallery

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  Following cell separation, cell suspension was stained with FITC-CD45(F10-89-4) and CL650-CD14(UCHM1) antibodies. All viable cells are gated in the analysis. Left panel: CD14+CD45+ cells before selection. Right panel: CD14+CD45+ cells after depletion. Human CD14 magnetic beads kit is tested using PBMC from three donors.

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Product Information

CD14 is a surface antigen expressed on monocyte/macrophage. It cooperates with other proteins to mediate innate immune response to bacterial LPS.  5%-10% human peripheral blood mononuclear cells (PBMC) express CD14. Human CD14 Magnetic Beads Kit is used for isolation or depletion of human CD14 monocytes/macrophages from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD14 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD14+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD14- cells remain in supernatant and can also be used for further applications.

Components

KMS005-10:  

  · MS001-10: 100µL 10mg/mL streptavidin magnetic beads  

  · MS65246-10: 100µL 0.1mg/mL Biotin-CD14 (clone: 26IC)  

KMS005-100:  

  · MS001-100: 1mL 10mg/mL streptavidin magnetic beads  

  · MS65246-100: 1mL 0.1mg/mL Biotin-CD14 (clone: 26IC)  

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide 

Reactivity

Human

Recommend usage

10µL Biotin-CD14 antibody and 10µL streptavidin beads for 1*10⁷ cells.

Cited in Article as

KMS005, Human CD14 Magnetic Beads Kit, Proteintech, IL, USA