Speedy™ Human CD141/Thrombomodulin One-Step ELISA Kit
Cat no : SE50059
Synonyms
THBD, Thrombomodulin, CD141, Fetomodulin, THRM
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD141/Thrombomodulin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Cell Lysate |
| Assay type | Sandwich |
| Sensitivity | 29.8 pg/mL |
| Range | 125-8000 pg/ml, 250-16000 pg/ml |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7056 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 109% | 101%-131% |
| Urine | 95% | 84%-100% |
| Cell lysate | 96% | 93%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4,059.3 | 221.3 | 5.5 |
| 2 | 8 | 1,041.6 | 60.1 | 5.8 |
| 3 | 8 | 306.5 | 8.8 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4,014.1 | 168.0 | 4.2 |
| 2 | 16 | 1,005.6 | 59.2 | 5.9 |
| 3 | 16 | 302.2 | 19.3 | 6.4 |
Background Information
Thrombomodulin (THBD, also known as CD141) is an endothelial-specific type I membrane glycoprotein of 105 kDa that binds thrombin, which plays an important role as a natural anticoagulant. It is expressed mostly on the surface of vascular endothelial cells, where it probably be involved in cell differentiation or inflammation. Soluble forms of CD141 have been found in plasma and urine. It has been reported that plasma CD141 is increased in patients with diabetes mellitus, particularly those with diabetic nephropathy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | THBD, Thrombomodulin, CD141, Fetomodulin, THRM |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.