Human CD163 ELISA Kit
Cat no : KE00190
Synonyms
CD163,CD163 molecule,Hemoglobin scavenger receptor
Validation Data Gallery
Product Information
KE00190 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD163 ELISA kit is to be used to detect and quantify protein levels of endogenous CD163. The CD163 ELISA kit is designed to measure human soluble CD163 in serum and plasma. The assay recognizes human CD163. An antibody specific for CD163 has been pre-coated onto the microwells. The CD163 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human CD163 is added to detect the captured human CD163 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD163 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 1.2 ng/mL |
| Range | 6.25 - 400 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9332 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 78%-112% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 44.3 | 1.2 | 2.8 |
| 2 | 20 | 64.2 | 1.7 | 2.7 |
| 3 | 20 | 195.9 | 6.7 | 3.4 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 50.9 | 3.7 | 7.2 |
| 2 | 24 | 76.4 | 6.2 | 8.1 |
| 3 | 24 | 247.5 | 21.3 | 8.6 |
Background Information
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily. It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages. CD163 mediates endocytosis of haptoglobin-haemoglobin complexes. The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD163,CD163 molecule,Hemoglobin scavenger receptor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
J Proteome Res Longitudinal Serum Proteomics Characterization of CD19-CAR-T Cell Therapy for B-Cell Malignancies | ||