Human CD2 ELISA Kit
Cat no : KE00408
Synonyms
CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, LFA-2
Validation Data Gallery
Product Information
KE00408 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD2 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD2. The assay recognizes human CD2. An antibody specific for human CD2 has been pre-coated onto the microwells. The human CD2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD2 is added to detect the captured human CD2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 9.8-625 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 914 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 92%-106% |
| Cell lysate | 91% | 87%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 321.0 | 9.4 | 2.9 |
| 2 | 20 | 79.5 | 1.3 | 1.6 |
| 3 | 20 | 23.6 | 1.4 | 5.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 331.4 | 13.5 | 4.1 |
| 2 | 24 | 81.3 | 2.9 | 3.5 |
| 3 | 24 | 26.7 | 0.9 | 3.3 |
Background Information
CD2 is a cell surface glycoprotein present on a majority of thymocytes, almost all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, LFA-2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.