Human CD200 ELISA Kit
Cat no : KE00323
Synonyms
CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein
Validation Data Gallery
Product Information
KE00323 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD200 ELISA kit is to be used to detect and quantify protein levels of endogenous CD200. The assay recognizes human CD200. An antibody specific for CD200 has been pre-coated onto the microwells. The CD200 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-CD200 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD200 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human plasma |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4345 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 93% | 81%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 786.3 | 15.3 | 1.9 |
| 2 | 20 | 170.9 | 4.9 | 2.9 |
| 3 | 20 | 40.9 | 3.3 | 8.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 798.6 | 34.5 | 4.3 |
| 2 | 24 | 166.5 | 10.8 | 6.5 |
| 3 | 24 | 33.9 | 2.7 | 7.9 |
Background Information
CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells. CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage. The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.