Speedy™ Human CD23 One-Step ELISA Kit
Cat no : SE50087
Synonyms
BLAST 2, BLAST-2, CD23A, CLEC4J, C-type lectin domain family 4 member J
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD23 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 27.2 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2208 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 86% | 81%-92% |
| Cell culture supernatant | 102% | 94%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,326.4 | 72.7 | 3.1 |
| 2 | 8 | 590.9 | 17.8 | 3.0 |
| 3 | 8 | 289.7 | 9.9 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,294.7 | 80.5 | 3.5 |
| 2 | 16 | 568.8 | 26.8 | 4.7 |
| 3 | 16 | 282.2 | 12.4 | 4.4 |
Background Information
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. CD23 can be cleaved into soluble fragment (soluble CD23, sCD23) of various sizes displaying pleiotropic biological activities. Increased plasma levels of sCD23 have been reported in allergic disorders, acute viral diseases, lepromatous leprosy, B-CLL, and inflammatory diseases like RA, SLE and sarcoidosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BLAST 2, BLAST-2, CD23A, CLEC4J, C-type lectin domain family 4 member J |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.