Speedy™ Human CD25/IL-2RA One-Step ELISA Kit
Cat no : SE50037
Synonyms
CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD25/IL-2RA One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3559 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 81%-112% |
| Cell culture supernatant | 107% | 95%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,024.0 | 45.6 | 4.5 |
| 2 | 8 | 236.1 | 3.5 | 1.5 |
| 3 | 8 | 122.8 | 2.5 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,067.1 | 84.6 | 7.9 |
| 2 | 16 | 244.8 | 9.9 | 4.0 |
| 3 | 16 | 127.3 | 5.7 | 4.5 |
Background Information
CD25, also known as the α chain of the IL-2 receptor (IL-2Rα), is a key regulator of T cell function. It is expressed in activated T cells and has been implicated in regulating various immune and autoimmune responses. Its deficiency has been associated with autoimmune phenomena including autoimmune enteropathy and bullous pemphigoid. The soluble CD25 can be found in the serum and may have implications for human disease in both cancer and chronic infection.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.