Human CD27 ELISA Kit
Cat no : KE00886
Synonyms
CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27
Validation Data Gallery
Product Information
KE00886 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD27 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD27. The assay recognizes human CD27. An antibody specific for human CD27 has been pre-coated onto the microwells. The human CD27 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD27 is added to detect the captured human CD27 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD27 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 939 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 79%-111% |
| Cell culture supernatant | 99% | 84%-112% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.19 | 0.06 | 1.08 |
| 2 | 8 | 1.25 | 0.05 | 3.81 |
| 3 | 8 | 0.39 | 0.01 | 3.72 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.36 | 0.21 | 4.00 |
| 2 | 16 | 1.30 | 0.06 | 4.68 |
| 3 | 16 | 0.40 | 0.02 | 4.86 |
Background Information
CD27 (also known as TNFRSF7) is a lymphocyte-specific member of the TNF/NGF-R family, which is found in NK, T, and B cell populations. CD27 is a T and B cell co-stimulatory molecule. Its activity is governed by the transient availability of its TNF-like ligand CD70 on lymphocytes and dendritic cells. This interaction may have a key role in controlling the size and function of antigen-primed lymphocyte populations. CD27 is also required for the effective generation and long-term maintenance of antigen-specific CD4+ and CD8+ T cell immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.