Human CD28 ELISA Kit
Cat no : KE00359
Synonyms
CD28,CD28 molecule,T-cell-specific surface glycopr
Validation Data Gallery
Product Information
KE00359 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD28 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD28. The assay recognizes human CD28. An antibody specific for human CD28 has been pre-coated onto the microwells. The human CD28 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD28 is added to detect the captured human CD28 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD28 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 47.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 940 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 86%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,819.2 | 111.2 | 3.9 |
| 2 | 20 | 655.4 | 18.8 | 2.9 |
| 3 | 20 | 173.1 | 11.1 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,635.7 | 141.8 | 5.4 |
| 2 | 24 | 664.8 | 29.7 | 4.5 |
| 3 | 24 | 154.5 | 13.7 | 8.8 |
Background Information
CD28 (T-cell-specific surface glycoprotein CD28), or T44 and Tp44, is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 may affect in vivo immune responses as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway. CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop. In addition, CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD28,CD28 molecule,T-cell-specific surface glycopr |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.