Human CD3 Delta ELISA Kit
Cat no : KE00632
Synonyms
CD3D, CD3-delta, T cell receptor T3 delta chain, T3D, T-cell receptor T3 delta chain
Validation Data Gallery
Product Information
KE00632 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD3 Delta ELISA kit is to be used to detect and quantify protein levels of endogenous human CD3 Delta. The assay recognizes human CD3 Delta. An antibody specific for human CD3 Delta has been pre-coated onto the microwells. The human CD3 Delta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD3 Delta is added to detect the captured human CD3 Delta protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD3 Delta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 915 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 97% | 78%-114% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 8.85 | 0.46 | 5.18 |
| 2 | 8 | 2.08 | 0.09 | 4.20 |
| 3 | 8 | 1.04 | 0.06 | 6.08 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.11 | 0.55 | 6.01 |
| 2 | 16 | 2.16 | 0.15 | 6.92 |
| 3 | 16 | 1.06 | 0.08 | 7.58 |
Background Information
CD3 Delta (CD3D) is a transmembrane protein primarily localized in the cell membrane of T cells. It is a crucial component of the T-cell receptor (TCR) complex, which plays a vital role in the activation of T cells. CD3 Delta is essential for the proper assembly and function of the TCR complex, facilitating the transmission of activation signals into the cytosol upon antigen recognition. Additionally, the TCR complex, including CD3 Delta, is a key target in cancer immunotherapy, where it can be engineered to enhance T-cell recognition and kill cancer cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD3D, CD3-delta, T cell receptor T3 delta chain, T3D, T-cell receptor T3 delta chain |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.