Speedy™ Human CD300A One-Step ELISA Kit
Cat no : SE50074
Synonyms
CD300 antigen-like family member A, CD300a molecule, CLM 8, CLM-8, CMRF 35 H9
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD300A One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 6.7 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11314 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 102% | 91%-112% |
| Urine | 109% | 107%-113% |
| Cell lysate | 81% | 77%-84% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,065.3 | 121.7 | 5.9 |
| 2 | 8 | 509.5 | 18.0 | 3.5 |
| 3 | 8 | 167.1 | 5.2 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,078.5 | 110.8 | 5.3 |
| 2 | 16 | 513.6 | 17.9 | 3.5 |
| 3 | 16 | 166.7 | 5.3 | 3.2 |
Background Information
CD300A is an inhibitory receptor expressed on various leukocytes. It plays a crucial role in modulating immune responses by recognizing phosphatidylserine (PS) and phosphatidylethanolamine (PE), two aminophospholipids exposed on the outer leaflet of dead and activated cells. This recognition helps regulate immune functions and the host's response to various disease states, such as infectious diseases, cancer, allergies, and chronic inflammatory diseases. CD300A delivers inhibitory signals through its immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which are phosphorylated upon ligand binding and recruit SH2-containing phosphatases, leading to the inhibition of downstream signaling pathways.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD300 antigen-like family member A, CD300a molecule, CLM 8, CLM-8, CMRF 35 H9 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.