Speedy™ Human CD39/ENTPD1 One-Step ELISA Kit
Cat no : SE50030
Synonyms
ENTPD1, ATP diphosphohydrolase, ATPDase, ATP-DPH, CD39
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD39/ENTPD1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 2.9 pg/mL |
| Range | 18.8-1200 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 953 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 84%-95% |
| Tissue lysate | 98% | 97%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 296.5 | 21.3 | 7.2 |
| 2 | 8 | 77.6 | 2.0 | 2.6 |
| 3 | 8 | 40.5 | 0.9 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 297.9 | 21.0 | 7.0 |
| 2 | 16 | 76.8 | 2.2 | 2.9 |
| 3 | 16 | 39.7 | 1.4 | 3.6 |
Background Information
ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1), also known as CD39 or NTPDase 1, is a cell surface enzyme that plays a crucial role in regulating extracellular nucleotide levels and immune responses. ENTPD1 is highly expressed on regulatory T cells (Tregs), where it contributes to their suppressive functions by depleting extracellular ATP and generating adenosine. It is a noncovalent tetrameric protein, and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers. Moreover, ENTPD1 is involved in the regulation of dendritic cell function and T cell activation. It modulates the local concentration of nucleotides and nucleosides, which in turn affects immune cell signaling and activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ENTPD1, ATP diphosphohydrolase, ATPDase, ATP-DPH, CD39 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.