Speedy™ Human CD40 One-Step ELISA Kit
Cat no : SE50046
Synonyms
Bp50, CD40L receptor, p50, TNFRSF5
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD40 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 20.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 958 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 89%-105% |
| Cell culture supernatant | 93% | 89%-101% |
| Urine | 100% | 86%-108% |
| Saliva | 89% | 75%-98% |
| Human milk | 100% | 86%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,690.8 | 41.0 | 1.5 |
| 2 | 8 | 662.4 | 5.8 | 0.9 |
| 3 | 8 | 330.7 | 6.1 | 1.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,689.1 | 81.8 | 3.0 |
| 2 | 16 | 664.2 | 12.5 | 1.9 |
| 3 | 16 | 327.5 | 9.0 | 2.7 |
Background Information
CD40 is a member of the tumor necrosis factor receptor superfamily. It is expressed in various immune cells, including B cells, macrophages, and dendritic cells. CD40 functions as a co-stimulatory receptor that interacts with its ligand, CD40L (CD154), primarily expressed on activated CD4+ T cells. This interaction is essential for T-cell activation, immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 signaling is mediated by recruitment of TRAFs (TNF receptor-associated factors) and NF-κB activation, supplemented by pathways such as PI3K/AKT, MAPKs, and JAK3/STATs. Additionally, circulating soluble CD40 levels are elevated in patients with chronic renal failure, chronic liver diseases, Alzheimer's disease, systemic sclerosis, and hematological malignancies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Bp50, CD40L receptor, p50, TNFRSF5 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.