Speedy™ Human CD40 One-Step ELISA Kit
Cat no : SE50046
Synonyms
Bp50, CD40L receptor, p50, TNFRSF5
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD40 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 20.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 958 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 89%-105% |
| Cell culture supernatant | 93% | 89%-101% |
| Urine | 100% | 86%-108% |
| Saliva | 89% | 75%-98% |
| Human milk | 100% | 86%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,690.8 | 41.0 | 1.5 |
| 2 | 8 | 662.4 | 5.8 | 0.9 |
| 3 | 8 | 330.7 | 6.1 | 1.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,689.1 | 81.8 | 3.0 |
| 2 | 16 | 664.2 | 12.5 | 1.9 |
| 3 | 16 | 327.5 | 9.0 | 2.7 |
Background Information
CD40 is a member of the tumor necrosis factor receptor superfamily. It is expressed in various immune cells, including B cells, macrophages, and dendritic cells. CD40 functions as a co-stimulatory receptor that interacts with its ligand, CD40L (CD154), primarily expressed on activated CD4+ T cells. This interaction is essential for T-cell activation, immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 signaling is mediated by recruitment of TRAFs (TNF receptor-associated factors) and NF-κB activation, supplemented by pathways such as PI3K/AKT, MAPKs, and JAK3/STATs. Additionally, circulating soluble CD40 levels are elevated in patients with chronic renal failure, chronic liver diseases, Alzheimer's disease, systemic sclerosis, and hematological malignancies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Bp50, CD40L receptor, p50, TNFRSF5 |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.