Human CD6 ELISA Kit
Cat no : KE00791
Synonyms
CD6 molecule, Soluble CD6, T12, T-cell differentiation antigen CD6, TP120
Validation Data Gallery
Product Information
KE00791 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD6 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD6. The assay recognizes human CD6. An antibody specific for human CD6 has been pre-coated onto the microwells. The human CD6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD6 is added to detect the captured human CD6 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 923 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 87%-107% |
| Cell lysate | 92% | 79%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,427.6 | 67.4 | 2.8 |
| 2 | 8 | 597.5 | 25.8 | 4.3 |
| 3 | 8 | 325.2 | 20.3 | 6.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,408.3 | 71.0 | 2.9 |
| 2 | 16 | 597.7 | 31.8 | 5.3 |
| 3 | 16 | 321.8 | 21.2 | 6.6 |
Background Information
CD6 is a lymphocyte surface accessory molecule that is associated with the T-cell receptor (TCR) complex in the immune synapse. It plays a key role in T-cell activation and proliferation by mediating cell-cell contacts and regulating T-cell responses. CD6 is involved in the stabilization and maturation of the immunological synapse, facilitating T-cell interactions with antigen-presenting cells (APCs) and subsequent T-cell proliferative responses. Additionally, CD6 has been shown to modulate T-cell activation by attenuating signaling cascades, acting as a rheostat-type signalosome that tunes T-cell activation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD6 molecule, Soluble CD6, T12, T-cell differentiation antigen CD6, TP120 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.