Human CD62L ELISA Kit
Cat no : KE00792
Synonyms
L-selectin, CD62 antigen-like family member L, gp90 MEL, gp90-MEL, hLHRc
Validation Data Gallery
Product Information
KE00792 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD62L ELISA kit is to be used to detect and quantify protein levels of endogenous human CD62L. The assay recognizes human CD62L. An antibody specific for human CD62L has been pre-coated onto the microwells. The human CD62L protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD62L is added to detect the captured human CD62L protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD62L ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6402 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 105% | 92%-124% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.78 | 0.41 | 8.54 |
| 2 | 8 | 1.19 | 0.10 | 8.60 |
| 3 | 8 | 0.65 | 0.03 | 3.99 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.63 | 0.43 | 9.26 |
| 2 | 16 | 1.19 | 0.08 | 6.69 |
| 3 | 16 | 0.65 | 0.02 | 3.21 |
Background Information
CD62L (L-selectin) is an adhesion molecule belonging to the selectin family, primarily expressed on the surface of white blood cells, and plays a role in various physiological and pathological processes, including lymphocyte homing, inflammatory responses, and immune cell positioning. It plays a crucial role in the homing process of white blood cells, helping them migrate from the bloodstream into lymphoid tissues. During inflammatory responses, CD62L mediates the initial adhesion of white blood cells to endothelial cells, facilitating their rolling and migration. Additionally, CD62L is a marker for central memory T cells, which have higher expression levels of CD62L and can rapidly relocate to lymph nodes and other sites for a swift secondary response.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | L-selectin, CD62 antigen-like family member L, gp90 MEL, gp90-MEL, hLHRc |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.