Human CD69 ELISA Kit
Cat no : KE00625
Synonyms
Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule
Validation Data Gallery
Product Information
KE00625 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD69 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD69. The assay recognizes human CD69. An antibody specific for human CD69 has been pre-coated onto the microwells. The human CD69 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD69 is added to detect the captured human CD69 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD69 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.4 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 969 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 90% | 85%-93% |
| Cell lysate | 77% | 74%-81% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,256.5 | 72.0 | 5.7 |
| 2 | 8 | 294.0 | 7.7 | 2.6 |
| 3 | 8 | 163.7 | 2.6 | 1.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,261.8 | 68.0 | 5.4 |
| 2 | 16 | 295.0 | 6.3 | 2.1 |
| 3 | 16 | 159.5 | 6.1 | 3.8 |
Background Information
CD69 is a type II C-type lectin receptor that serves as an early activation marker for lymphocytes. It is rapidly expressed on the surface of T cells, B cells, and natural killer (NK) cells following activation. CD69 plays a crucial role in lymphocyte proliferation and functions as a signal-transmitting receptor, influencing cell cycle progression and cytokine production. It also regulates the differentiation of T helper (Th) subsets, including Th1, Th2, Th17, and regulatory T cells (Tregs), by modulating intracellular signaling pathways and cytokine secretion.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.