Human CD72 ELISA Kit
Cat no : KE00695
Synonyms
B-cell differentiation antigen CD72, CD72 molecule, CD72b, Lyb 2, LYB2
Validation Data Gallery
Product Information
KE00695 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD72 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD72. The assay recognizes human CD72. An antibody specific for human CD72 has been pre-coated onto the microwells. The human CD72 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD72 is added to detect the captured human CD72 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD72 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 971 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 95% | 84%-103% |
| Cell lysate | 108% | 99%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 517.7 | 15.8 | 3.1 |
| 2 | 8 | 115.2 | 2.8 | 2.4 |
| 3 | 8 | 60.2 | 2.8 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 512.7 | 17.4 | 3.4 |
| 2 | 16 | 119.1 | 6.9 | 5.8 |
| 3 | 16 | 59.5 | 3.1 | 5.3 |
Background Information
CD72 is a regulatory receptor expressed on B cells that plays a crucial role in maintaining self-tolerance and modulating B cell responses. It is a member of the C-type lectin superfamily. CD72 contains an immune receptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic tail, which recruits the tyrosine phosphatase SHP-1 to regulate B cell receptor (BCR) signaling negatively. Additionally, elevated levels of soluble CD72 (sCD72) have been reported in the serum of SLE patients, correlating with disease severity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B-cell differentiation antigen CD72, CD72 molecule, CD72b, Lyb 2, LYB2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.