Human CD79a ELISA Kit
Cat no : KE00659
Synonyms
B-cell antigen receptor complex-associated protein alpha chain, MB 1, MB 1 membrane glycoprotein, MB1, MB-1 membrane glycoprotein
Validation Data Gallery
Product Information
KE00659 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD79a ELISA kit is to be used to detect and quantify protein levels of endogenous human CD79a. The assay recognizes human CD79a. An antibody specific for human CD79a has been pre-coated onto the microwells. The human CD79a protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD79a is added to detect the captured human CD79a protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD79a ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 973 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 96% | 83%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 792.6 | 36.5 | 4.6 |
| 2 | 8 | 179.1 | 3.8 | 2.1 |
| 3 | 8 | 58.1 | 2.0 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 786.5 | 51.4 | 6.5 |
| 2 | 16 | 182.6 | 4.8 | 2.6 |
| 3 | 16 | 58.9 | 1.9 | 3.2 |
Background Information
CD79a is a type I transmembrane glycoprotein, primarily localized in the cell membrane of B cells and a crucial component of the B cell receptor (BCR) complex. It forms a disulfide-linked heterodimer with CD79b (Igβ), which non-covalently associates with membrane-bound immunoglobulins on B cells to form the BCR. This complex is essential for B cell antigen recognition, activation, proliferation, and differentiation. Moreover, the CD79a expression level is associated with the pathogenesis of B-cell acute lymphoblastic leukemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B-cell antigen receptor complex-associated protein alpha chain, MB 1, MB 1 membrane glycoprotein, MB1, MB-1 membrane glycoprotein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.