Human CD79b ELISA Kit
Cat no : KE00749
Synonyms
B29, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig beta, IGB
Validation Data Gallery
Product Information
KE00749 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD79b ELISA kit is to be used to detect and quantify protein levels of endogenous human CD79b. The assay recognizes human CD79b. An antibody specific for human CD79b has been pre-coated onto the microwells. The human CD79b protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD79b is added to detect the captured human CD79b protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD79b ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 3.13-200 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 974 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 84%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 112.9 | 2.9 | 2.5 |
| 2 | 8 | 28.2 | 0.7 | 2.4 |
| 3 | 8 | 14.3 | 0.5 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 108.5 | 5.2 | 4.8 |
| 2 | 16 | 29.3 | 1.3 | 4.4 |
| 3 | 16 | 14.7 | 0.6 | 4.1 |
Background Information
CD79b is a member of the Ig-Superfamily. It is a crucial component of the B cell antigen receptor (BCR) complex, essential for B cell activation and function. CD79b forms a heterodimer with CD79a (Igα) and is involved in BCR signaling, which is critical for B cell development, proliferation, and differentiation. The N-terminal tyrosine kinase structure domain of CD79b can recruit and activate downstream signaling molecules such as Syk, playing a core role in B cell signaling. Moreover, mutations in CD79b can lead to constitutive activation of downstream signaling pathways, contributing to B cell lymphoma development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B29, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig beta, IGB |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.