Human CD89 ELISA Kit
Cat no : KE00881
Synonyms
FCAR, IgA Fc receptor
Validation Data Gallery
Product Information
KE00881 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD89 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD89. The assay recognizes human CD89. An antibody specific for human CD89 has been pre-coated onto the microwells. The human CD89 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD89 is added to detect the captured human CD89 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD89 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 8.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2204 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 93% | 90%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 988.2 | 12.2 | 1.2 |
| 2 | 8 | 268.6 | 6.4 | 2.4 |
| 3 | 8 | 134.7 | 7.2 | 5.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,003.0 | 44.2 | 4.4 |
| 2 | 16 | 261.7 | 14.6 | 5.6 |
| 3 | 16 | 132.8 | 7.6 | 5.7 |
Background Information
FcαRI (CD89) is a member of the Fc receptor immunoglobulin superfamily, constitutively expressed on cells of the myeloid lineage, like neutrophils, eosinophils, monocytes, macrophages, and platelets. CD89 expression and activation are increased in human blood phagocytes following bacterial infection, together with increased expression and association of FcRγ with CD89. CD89 binds both subclasses of IgA (IgA1 and IgA2) with moderate affinity, but also binds other molecules such as TfR1, C-reactive protein, and bacterial ligands. CD89 represents an effective target molecule for immunotherapy mediated by bispecific antibodies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FCAR, IgA Fc receptor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.