Speedy™ Human CD93 One-Step ELISA Kit
Cat no : SE50079
Synonyms
C1QR1, C1qR, C1qR(p), C1qRp, Matrix-remodeling-associated protein 4
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD93 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 12.9 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22918 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 111% | 105%-123% |
| Cell culture supernatant | 107% | 100%-111% |
| Urine | 110% | 101%-123% |
| Saliva | 110% | 101%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,596.2 | 54.2 | 2.1 |
| 2 | 8 | 638.7 | 14.2 | 2.2 |
| 3 | 8 | 327.4 | 7.7 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,560.1 | 54.2 | 2.1 |
| 2 | 16 | 635.8 | 13.9 | 2.2 |
| 3 | 16 | 322.5 | 8.2 | 2.5 |
Background Information
CD93 is a transmembrane glycoprotein primarily expressed on endothelial cells, monocytes, and macrophages. It regulates immune cell activation and differentiation, particularly in the context of inflammation and immune cell activation. Reports indicate an association between CD93 and diseases of which inflammation is a fundamental characteristic, such as asthma, bronchitis, and arthritis. Additionally, it has been reported that increased levels of this soluble form of CD93 are found in plasma from patients with myocardial infarction.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C1QR1, C1qR, C1qR(p), C1qRp, Matrix-remodeling-associated protein 4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.