Human CD97 ELISA Kit
Cat no : KE00712
Synonyms
ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta, CD97 antigen
Validation Data Gallery
Product Information
KE00712 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD97 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD97. The assay recognizes human CD97. An antibody specific for human CD97 has been pre-coated onto the microwells. The human CD97 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD97 is added to detect the captured human CD97 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD97 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 20.5 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 976 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 93% | 86%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,308.6 | 146.2 | 6.3 |
| 2 | 8 | 616.8 | 34.7 | 5.6 |
| 3 | 8 | 192.1 | 12.9 | 6.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,359.8 | 237.0 | 10.0 |
| 2 | 16 | 627.3 | 51.2 | 8.2 |
| 3 | 16 | 194.1 | 17.0 | 8.8 |
Background Information
CD97 is a member of the seven-transmembrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF). It is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein. It plays a vital role in cell adhesion, migration, and cell connection regulation. CD97 is expressed on T-cells, but rarely on B-cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta, CD97 antigen |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.