Human CD99 ELISA Kit
Cat no : KE00589
Synonyms
CD99, 12E7, CD99 antigen, CD99 molecule, E2 antigen
Validation Data Gallery
Product Information
KE00589 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD99 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD99. The assay recognizes human CD99. An antibody specific for human CD99 has been pre-coated onto the microwells. The human CD99 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD99 is added to detect the captured human CD99 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD99 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4267 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 104% | 99%-112% |
| Cell lysate | 89% | 87%-93% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.03 | 0.35 | 3.54 |
| 2 | 8 | 2.71 | 0.16 | 5.92 |
| 3 | 8 | 1.39 | 0.09 | 6.56 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 10.00 | 0.44 | 4.36 |
| 2 | 16 | 2.68 | 0.17 | 6.37 |
| 3 | 16 | 1.41 | 0.11 | 7.99 |
Background Information
CD99 is a highly O-glycosylated type I transmembrane protein that is mainly expressed in cells of the hematopoietic system and at the intercellular borders of endothelial cells. CD99 is essential for transendothelial migration (TEM) of monocytes, neutrophils, and lymphocytes. It is frequently overexpressed in many types of tumors, particularly pediatric tumors including Ewing sarcoma and specific subtypes of leukemia. CD99 is also a novel marker of acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) stem cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD99, 12E7, CD99 antigen, CD99 molecule, E2 antigen |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.