Human CDK2 ELISA Kit
Cat no : KE00451
Synonyms
CDK2,CDKN2,Cell division protein kinase 2,Cyclin d
Validation Data Gallery
Product Information
KE00451 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CDK2 ELISA kit is to be used to detect and quantify protein levels of endogenous human CDK2. The assay recognizes human CDK2. An antibody specific for human CDK2 has been pre-coated onto the microwells. The human CDK2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CDK2 is added to detect the captured human CDK2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CDK2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 45.9 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1017 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 81% | 76%-85% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4,751.7 | 331.1 | 7.0 |
| 2 | 8 | 1,518.2 | 45.5 | 3.0 |
| 3 | 8 | 424.1 | 29.9 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4,950.2 | 393.3 | 7.9 |
| 2 | 16 | 1,483.6 | 60.6 | 4.1 |
| 3 | 16 | 413.0 | 34.5 | 8.3 |
Background Information
Cyclin-dependent kinase 2 (CDK2) is also named as CDKN2. CDK2 is a core cell-cycle kinase that phosphorylates many substrates to drive progression through the cell cycle. CDK2 is hyperactivated in multiple cancers and is therefore an attractive therapeutic target. CDK4/6-cyclin D complexes phosphorylate and inactivate the retinoblastoma protein (Rb1), liberating E2F to promote transcription of cyclins E and A, which activate CDK2. Activated CDK2 hyperphosphorylates Rb1, establishing a potent positive feedback loop that ensures the continued expression of cyclins E and A, as well as other proteins that are essential for S phase.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CDK2,CDKN2,Cell division protein kinase 2,Cyclin d |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.