Speedy™ Human CEA/CEACAM5 One-Step ELISA Kit
Cat no : SE50050
Synonyms
CEA, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e, CEACAM5
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CEA/CEACAM5 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1048 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 112% | 103%-117% |
| Saliva | 95% | 90%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 474.2 | 13.4 | 2.8 |
| 2 | 8 | 109.9 | 2.6 | 2.4 |
| 3 | 8 | 55.7 | 1.7 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 474.5 | 11.2 | 2.4 |
| 2 | 16 | 109.7 | 2.6 | 2.4 |
| 3 | 16 | 54.0 | 2.5 | 4.6 |
Background Information
CEACAM5, also known as carcinoembryonic antigen (CEA) or CD66e, is a member of the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding. CEACAM5 is overexpressed in a wide variety of human cancers, including colon, breast, and lung. It inhibits anoikis and plays a role in tumorigenesis and metastasis. CEACAM5 is not usually present in the blood of healthy adults. However, it has been found that serum from people with colorectal, gastric, pancreatic, lung, and breast carcinoma has higher levels of CEACAM5 than healthy people. CEACAM5 is a tumor marker and is routinely exploited for diagnosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CEA, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e, CEACAM5 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.