Human CEACAM3 ELISA Kit
Cat no : KE00677
Synonyms
Carcinoembryonic antigen CGM1, Carcinoembryonic antigen-related cell adhesion molecule 3, CD66D, CGM1, W264
Validation Data Gallery
Product Information
KE00677 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CEACAM3 ELISA kit is to be used to detect and quantify protein levels of endogenous human CEACAM3. The assay recognizes human CEACAM3. An antibody specific for human CEACAM3 has been pre-coated onto the microwells. The human CEACAM3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CEACAM3 is added to detect the captured human CEACAM3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CEACAM3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1084 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 123% | 116%-130% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.30 | 0.17 | 3.15 |
| 2 | 8 | 1.26 | 0.06 | 4.38 |
| 3 | 8 | 0.60 | 0.01 | 1.83 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.31 | 0.23 | 4.42 |
| 2 | 16 | 1.23 | 0.06 | 4.67 |
| 3 | 16 | 0.62 | 0.03 | 4.25 |
Background Information
CEA cell adhesion molecule 3 (CEACAM3) is an immunoglobulin-related glycoprotein that plays a crucial role in the innate immune response. It is expressed exclusively on granulocytes and is involved in the opsonin-independent recognition and elimination of specific Gram-negative bacterial pathogens. Unlike other CEACAM family members, CEACAM3 does not support cell-cell adhesion but functions as a receptor for pathogen recognition and elimination. Additionally, CEACAM3 has been suggested to have potential applications in cancer immunotherapy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Carcinoembryonic antigen CGM1, Carcinoembryonic antigen-related cell adhesion molecule 3, CD66D, CGM1, W264 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.