Human CEACAM6 ELISA Kit
Cat no : KE00320
Synonyms
Carcinoembryonic antigen-related cell adhesion molecule 6, CD66c, CEAL, NCA, Non-specific crossreacting antigen
Validation Data Gallery
Product Information
KE00320 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CEACAM6 ELISA kit is to be used to detect and quantify protein levels of endogenous CEACAM6. The assay recognizes human CEACAM6. An antibody specific for CEACAM6 has been pre-coated onto the microwells. The CEACAM6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-CEACAM6 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CEACAM6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum，Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4680 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 87%-122% |
| Cell culture supernatants | 109% | 94%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 213.6 | 8.2 | 3.8 |
| 2 | 20 | 50 | 3.4 | 6.8 |
| 3 | 20 | 11.5 | 0.8 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 203.8 | 10.9 | 5.4 |
| 2 | 24 | 46.9 | 2.8 | 6.0 |
| 3 | 24 | 11.4 | 0.5 | 4.3 |
Background Information
CEACAM6, also known as CD66c, is a glycosylphosphatidylinositol (GPI)-linked cell surface protein that belongs to the CEACAM family of the immunoglobulin superfamily. It is normally expressed on the surface of myeloid and epithelial surfaces and upregulated preferentially at the apical/luminal membranes of many tumors. CEACAM6 plays an important role in cell adhesion, proliferation, tumorigenesis, metastasis, and immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Carcinoembryonic antigen-related cell adhesion molecule 6, CD66c, CEAL, NCA, Non-specific crossreacting antigen |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.