Human CEACAM8/CD66b ELISA Kit
Cat no : KE00306
Synonyms
CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b
Validation Data Gallery
Product Information
KE00306 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CEACAM8/CD66b ELISA kit is to be used to detect and quantify protein levels of endogenous human CEACAM8/CD66b. The assay recognizes human CEACAM8/CD66b. An antibody specific for human CEACAM8/CD66b has been pre-coated onto the microwells. The human CEACAM8/CD66b protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CEACAM8/CD66b is added to detect the captured human CEACAM8/CD66b protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CEACAM8/CD66b ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 14.3 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1088 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 80%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3,048.4 | 99.5 | 3.3 |
| 2 | 20 | 743.6 | 23.1 | 3.1 |
| 3 | 20 | 191.2 | 13.3 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3,055.9 | 101.5 | 3.3 |
| 2 | 24 | 709.0 | 18.7 | 2.6 |
| 3 | 24 | 180.5 | 8.5 | 4.7 |
Background Information
A glycosylphosphatidyl-inositol-(GPI)-anchored glycoprotein, carcinoembryonic antigen (CEA)-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, NCA-95 and CD67, was identified in human neutrophils. It is solely expressed by granulocytes, of which 95% are polymorphonuclear neutrophils (PMN). CEACAM8 is cell-adhesion proteins on neutrophils that belong to the human carcinoembryonic antigen (CEA) family. CEA family members are believed to function as intercellular adhesion molecules in vitro. CEACAM8 has been shown to adhere to the closely related molecule CEACAM6 (NCA-50/90 and CD66c), suggesting a role in the interaction between granulocytes or between granulocytes and epithelial cells expressing CEACAM6 in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.