Human CEBPB ELISA Kit
Cat no : KE00461
Synonyms
CEBPB,C EBP beta,C/EBP beta,C/EBP related protein
Validation Data Gallery
Product Information
KE00461 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CEBPB ELISA kit is to be used to detect and quantify protein levels of endogenous human CEBPB. The assay recognizes human CEBPB. An antibody specific for human CEBPB has been pre-coated onto the microwells. The human CEBPB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CEBPB is added to detect the captured human CEBPB protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CEBPB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1051 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 102% | 95%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 984.6 | 51.2 | 5.2 |
| 2 | 8 | 254.7 | 2.6 | 1.0 |
| 3 | 8 | 123.3 | 2.9 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 986.5 | 44.5 | 4.5 |
| 2 | 16 | 244.7 | 13.7 | 5.6 |
| 3 | 16 | 120.0 | 6.6 | 5.5 |
Background Information
CCAAT/enhancer binding protein β (C/EBPβ), originally identified as a nuclear factor interleukin-6 (NF-IL6), plays a role in regulating inflammatory responses. C/EBPβ binds to the IL-1 response element within the promoter region of IL-6 and promotes the transcription of IL-6 and IL-8. C/EBPβ can directly interact with the Rel homologous domain situated between the nuclear factor κB (NF-κB) subunits p50, p65, and C-REL, thereby stabilizing NF-κB and synergistically activating the expression of IL-6 and IL-8. C/EBPβ can also regulate NF-κB by directly inactivating the inhibitor IκBα. C/EBPβ mediates the balance between anti-inflammatory and pro-inflammatory signals during tissue repair.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CEBPB,C EBP beta,C/EBP beta,C/EBP related protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.