Speedy™ Human CEBPB One-Step ELISA Kit
Cat no : SE50062
Synonyms
C EBP beta, C/EBP beta, C/EBP related protein 2, C/EBPbeta, CCAAT/enhancer-binding protein beta
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CEBPB One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 12.4 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1051 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 96%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,332.7 | 25.0 | 1.1 |
| 2 | 8 | 498.1 | 7.1 | 1.4 |
| 3 | 8 | 164.1 | 2.4 | 1.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,333.0 | 39.3 | 1.7 |
| 2 | 16 | 499.9 | 9.8 | 2.0 |
| 3 | 16 | 162.4 | 3.1 | 1.9 |
Background Information
CCAAT/enhancer binding protein β (C/EBPβ), originally identified as a nuclear factor interleukin-6 (NF-IL6), plays a role in regulating inflammatory responses. C/EBPβ binds to the IL-1 response element within the promoter region of IL-6 and promotes the transcription of IL-6 and IL-8. C/EBPβ can directly interact with the Rel homologous domain situated between the nuclear factor κB (NF-κB) subunits p50, p65, and C-REL, thereby stabilizing NF-κB and synergistically activating the expression of IL-6 and IL-8. C/EBPβ can also regulate NF-κB by directly inactivating the inhibitor IκBα. C/EBPβ mediates the balance between anti-inflammatory and pro-inflammatory signals during tissue repair.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C EBP beta, C/EBP beta, C/EBP related protein 2, C/EBPbeta, CCAAT/enhancer-binding protein beta |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.