Human CKAP4 ELISA Kit
Cat no : KE00620
Synonyms
63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63, p63
Validation Data Gallery
Product Information
KE00620 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CKAP4 ELISA kit is to be used to detect and quantify protein levels of endogenous human CKAP4. The assay recognizes human CKAP4. An antibody specific for human CKAP4 has been pre-coated onto the microwells. The human CKAP4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CKAP4 is added to detect the captured human CKAP4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CKAP4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10970 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 74%-104% |
| Cell lysate | 100% | 83%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 552.2 | 44.9 | 8.1 |
| 2 | 8 | 127.9 | 12.5 | 9.8 |
| 3 | 8 | 76.9 | 5.6 | 7.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 563.2 | 54.1 | 9.6 |
| 2 | 16 | 128.9 | 11.6 | 9.0 |
| 3 | 16 | 72.7 | 7.1 | 9.8 |
Background Information
CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation. In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2, and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63, p63 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.