Human CLEC4M/DC-SIGNR ELISA Kit
Cat no : KE00674
Synonyms
CLEC4M, CD209 antigen like protein 1, CD209 antigen-like protein 1, CD209L, CD209L1
Validation Data Gallery
Product Information
KE00674 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CLEC4M/DC-SIGNR ELISA kit is to be used to detect and quantify protein levels of endogenous human CLEC4M/DC-SIGNR. The assay recognizes human CLEC4M/DC-SIGNR. An antibody specific for human CLEC4M/DC-SIGNR has been pre-coated onto the microwells. The human CLEC4M/DC-SIGNR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CLEC4M/DC-SIGNR is added to detect the captured human CLEC4M/DC-SIGNR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CLEC4M/DC-SIGNR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10332 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 88%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,690.2 | 60.0 | 3.5 |
| 2 | 8 | 377.7 | 20.8 | 5.5 |
| 3 | 8 | 210.7 | 7.9 | 3.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,808.8 | 166.7 | 9.2 |
| 2 | 16 | 381.1 | 16.7 | 4.4 |
| 3 | 16 | 211.6 | 6.2 | 3.0 |
Background Information
C-type lectin domain family 4 member M (CLEC4M, also known as DC-SIGNR) is a C-type lectin that functions in cell adhesion and pathogen recognition. It is predominantly found on the sinusoidal endothelial cells in the liver and lymph nodes, as well as in the placental endothelium. CLEC4M is also involved in the innate immune system and recognizes diverse pathogens, ranging from viruses to parasites, that have a tremendous impact on public health. Additionally, expression of CLEC4M in serum and cancer tissues may affect the survival time for colon cancer patients.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLEC4M, CD209 antigen like protein 1, CD209 antigen-like protein 1, CD209L, CD209L1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.