Human CSF1R/CD115 ELISA Kit
Cat no : KE00618
Synonyms
CSF1R, C FMS, CD115, CSF 1 R, CSF-1 receptor
Validation Data Gallery
Product Information
KE00618 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CSF1R ELISA kit is to be used to detect and quantify protein levels of endogenous human CSF1R. The assay recognizes human CSF1R. An antibody specific for human CSF1R has been pre-coated onto the microwells. The human CSF1R protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CSF1R is added to detect the captured human CSF1R protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CSF1R/CD115 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1436 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 93%-113% |
| Cell lysate | 95% | 92%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,819.3 | 110.5 | 6.1 |
| 2 | 8 | 364.3 | 8.3 | 2.3 |
| 3 | 8 | 194.7 | 4.5 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,782.6 | 107.4 | 6.0 |
| 2 | 16 | 365.2 | 8.0 | 2.2 |
| 3 | 16 | 187.7 | 9.3 | 5.0 |
Background Information
CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R is ubiquitously expressed in macrophages, microglia, osteoclasts, and myeloid dendritic cells, but is rarely expressed in hematopoietic stem cells.CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CSF1R, C FMS, CD115, CSF 1 R, CSF-1 receptor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.