Speedy™ Human CSF2RA/CD116 One-Step ELISA Kit
Cat no : SE50026
Synonyms
CD116, CSF2R, CSF2RA, GM CSF R alpha, GMCSFR
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CSF2RA/CD116 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lystae |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1438 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 90% | 83%-96% |
| Cell lysate | 86% | 82%-89% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,350.8 | 87.2 | 6.5 |
| 2 | 8 | 323.9 | 19.1 | 5.9 |
| 3 | 8 | 154.1 | 8.9 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,350.2 | 88.4 | 6.5 |
| 2 | 16 | 324.5 | 15.8 | 4.9 |
| 3 | 16 | 156.3 | 9.0 | 5.7 |
Background Information
CSF2RA, also known as CD116, is the alpha subunit of the heterodimeric receptor for colony-stimulating factor 2 (GM-CSF). CSF2RA plays a crucial role in the proliferation, differentiation, and functional activation of hematopoietic cells. CSF2RA is over-expressed in certain cancers and most immune cells, which may contribute to the activation of the immune system. The CSF2RA deletion in mice has been shown to attenuate the pro-inflammatory response and improve survival in the context of acute lung injury (ALI) induced by ricin toxin (RT).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD116, CSF2R, CSF2RA, GM CSF R alpha, GMCSFR |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.