Human CST5 ELISA Kit
Cat no : KE00753
Synonyms
Cystatin 5, cystatin D
Validation Data Gallery
Product Information
KE00753 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CST5 ELISA kit is to be used to detect and quantify protein levels of endogenous human CST5. The assay recognizes human CST5. An antibody specific for human CST5 has been pre-coated onto the microwells. The human CST5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CST5 is added to detect the captured human CST5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CST5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Saliva, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.15 ng/mL |
| Range | 0.94-60 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1473 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Saliva | 98% | 91%-104% |
| Tissue lysate | 92% | 87%-99% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 25.00 | 2.28 | 9.13 |
| 2 | 8 | 6.42 | 0.32 | 5.06 |
| 3 | 8 | 3.07 | 0.28 | 9.02 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.12 | 2.31 | 9.19 |
| 2 | 16 | 6.27 | 0.42 | 6.70 |
| 3 | 16 | 3.20 | 0.32 | 10.08 |
Background Information
CST5 (cystatin D), it is expected to be located in cytoplasmic and extracellular, and the protein is restrictively expressed at salivary gland. Human cystatin D is a novel member of the cystatin superfamily of cysteine proteinase inhibitors present in saliva and tears. The inhibitory properties displayed by cystatin D suggest that it has a function in saliva as inhibitor of either endogenous or exogenous enzymes with cathepsin S- or H-like properties (PMID: 8083219). The calculated molecular weight of CST5 is 16 kDa.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cystatin 5, cystatin D |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.