Human CTLA-4/CD152 ELISA Kit
Cat no : KE00448
Synonyms
CTLA4,CD152,CELIAC3,CTLA 4,Cytotoxic T-lymphocyte
Validation Data Gallery
Product Information
KE00448 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CTLA-4/CD152 ELISA kit is to be used to detect and quantify protein levels of endogenous human CTLA-4/CD152. The assay recognizes human CTLA-4/CD152. An antibody specific for human CTLA-4/CD152 has been pre-coated onto the microwells. The human CTLA-4/CD152 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CTLA-4/CD152 is added to detect the captured human CTLA-4/CD152 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CTLA-4/CD152 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plsama, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 3.7 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1493 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 83% | 78%-87% |
| Urine | 99% | 92%-105% |
| Cell lysate | 112% | 108%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 742.0 | 34.2 | 4.6 |
| 2 | 8 | 176.9 | 4.5 | 2.6 |
| 3 | 8 | 95.0 | 2.4 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 729.6 | 46.0 | 6.3 |
| 2 | 16 | 173.5 | 6.5 | 3.7 |
| 3 | 16 | 92.1 | 5.2 | 5.6 |
Background Information
CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CTLA4,CD152,CELIAC3,CTLA 4,Cytotoxic T-lymphocyte |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.