Human CTNNBIP1 ELISA Kit
Cat no : KE00430
Synonyms
CTNNBIP1,Beta-catenin-interacting protein 1,ICAT,I
Validation Data Gallery
Product Information
KE00430 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CTNNBIP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human CTNNBIP1. The assay recognizes human CTNNBIP1. An antibody specific for human CTNNBIP1 has been pre-coated onto the microwells. The human CTNNBIP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CTNNBIP1 is added to detect the captured human CTNNBIP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CTNNBIP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 4.6 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56998 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 95%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,240.7 | 58.2 | 4.7 |
| 2 | 8 | 263.9 | 9.4 | 3.6 |
| 3 | 8 | 41.8 | 3.6 | 8.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,259.7 | 49.6 | 3.9 |
| 2 | 16 | 278.6 | 18.6 | 6.7 |
| 3 | 16 | 41.5 | 4.3 | 10.4 |
Background Information
Catenin beta interacting protein 1 (CTNNBIP1), also known as ICAT or an inhibitor of β-catenin, prevents β-catenin from forming a complex with the T-cell factor/lymphoid enhancer factor (TCF/LEF) and thus inactivates the transcription of Wnt target genes, thereby negatively regulating the Wnt/β-catenin pathway . Furthermore, several studies have suggested CTNNBIP1 as a tumor suppressor in cancers of the colorectum , breasts , cervix and stomach. The CTNNBIP1 function as tumor suppressor gene or oncogene in different types of cancer is controversial.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CTNNBIP1,Beta-catenin-interacting protein 1,ICAT,I |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.