Human CTR9 ELISA Kit
Cat no : KE00960
Synonyms
KIAA0155, p150, RNA polymerase-associated protein CTR9 homolog, SH2 domain-binding protein 1, SH2BP1
Validation Data Gallery
Product Information
KE00960 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CTR9 ELISA kit is to be used to detect and quantify protein levels of endogenous human CTR9. The assay recognizes human CTR9. An antibody specific for human CTR9 has been pre-coated onto the microwells. The human CTR9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CTR9 is added to detect the captured human CTR9 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CTR9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.10 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9646 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 83% | 70%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 28.03 | 1.12 | 3.98 |
| 2 | 8 | 6.42 | 0.49 | 7.64 |
| 3 | 8 | 3.24 | 0.27 | 8.37 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.87 | 2.49 | 9.61 |
| 2 | 16 | 6.17 | 0.51 | 8.24 |
| 3 | 16 | 3.09 | 0.28 | 8.95 |
Background Information
CTR9 is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | KIAA0155, p150, RNA polymerase-associated protein CTR9 homolog, SH2 domain-binding protein 1, SH2BP1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.