Human CTRL ELISA Kit
Cat no : KE00883
Synonyms
chymotrypsin like, Chymotrypsin-like protease CTRL-1, CTRL1, EC:3.4.21.-
Validation Data Gallery
Product Information
KE00883 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CTRL ELISA kit is to be used to detect and quantify protein levels of endogenous human CTRL. The assay recognizes human CTRL. An antibody specific for human CTRL has been pre-coated onto the microwells. The human CTRL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CTRL is added to detect the captured human CTRL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CTRL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1506 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 96% | 93%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,339.6 | 38.6 | 2.9 |
| 2 | 8 | 323.1 | 18.6 | 5.8 |
| 3 | 8 | 158.3 | 5.6 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,339.8 | 47.7 | 3.6 |
| 2 | 16 | 317.4 | 20.9 | 6.6 |
| 3 | 16 | 161.3 | 7.3 | 4.5 |
Background Information
CTRL (Copper Transporter) is a transmembrane protein belonging to the CTR family, primarily responsible for the uptake of extracellular copper ions and their distribution within the cell. It is highly conserved across various species and is crucial for maintaining cellular copper homeostasis. CTRL captures copper ions through its N-terminal extracellular domain and transports them into the cell, ensuring their proper distribution within the cell. Dysfunction of CTRL is associated with a variety of diseases, including neurological disorders, cardiovascular diseases, and resistance to platinum-based chemotherapeutic drugs.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | chymotrypsin like, Chymotrypsin-like protease CTRL-1, CTRL1, EC:3.4.21.- |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.