Human CXCL1 ELISA Kit
Cat no : KE00133
Synonyms
C X C motif chemokine 1, C-X-C motif chemokine 1, CXCL 1, GRO-alpha(4-73), GRO-alpha(5-73)
Validation Data Gallery
Product Information
KE00133 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL1 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL1. The assay recognizes human CXCL1. An antibody specific for CXCL1 has been pre-coated onto the microwells. The CXCL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CXCL1 is added to detect the captured human CXCL1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CXCL1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 32.4 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2919 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 90% | 83%-106% |
| cell culture supernatants | 93% | 78%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,784.4 | 89.6 | 5.0 |
| 2 | 20 | 474.2 | 11.8 | 2.5 |
| 3 | 20 | 115.2 | 1.9 | 1.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,812.5 | 121.9 | 6.7 |
| 2 | 24 | 472.1 | 10.3 | 2.2 |
| 3 | 24 | 112.8 | 6.9 | 6.1 |
Background Information
CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C X C motif chemokine 1, C-X-C motif chemokine 1, CXCL 1, GRO-alpha(4-73), GRO-alpha(5-73) |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Commun Histone lactylation increases CXCL1 expression for neutrophil infiltration and immune escape in pancreatic cancer. | ||
J Nanobiotechnology Hypoxic glioma-derived exosomal miR-25-3p promotes macrophage M2 polarization by activating the PI3K-AKT-mTOR signaling pathway | ||
Theranostics Discovery of indole analogue Tc3 as a potent pyroptosis inducer and identification of its combination strategy against hepatic carcinoma | ||
Cell Rep Med Modeling antithymocyte globulin-induced microvasculopathy using human iPSC-derived vascularized liver organoids. | ||
Phytother Res Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke-induced airway inflammation. | ||
Cell Rep Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer |