Human CXCL1 ELISA Kit1 Publications

Catalog number: KE00133

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Freight/Packing £00.00

Size: 09µ/gram

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Product name:

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants

Assay type:

32.4 pg/mL

62.5-4000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Human plasma 90% 77%-102%
cell culture supernatants 93% 78%-111%

Samplenmean ( pg/mL)SDCV%
1 20 1,784.4 89.6 5.0
2 20 474.2 11.8 2.5
3 20 115.2 1.9 1.6

Samplenmean ( pg/mL)SDCV%
1 24 1,812.5 121.9 6.7
2 24 472.1 10.3 2.2
3 24 112.8 6.9 6.1

Product overview:
KE00133 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL1 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL1. The assay recognizes human CXCL1. An antibody specific for CXCL1 has been pre-coated onto the microwells. The CXCL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CXCL1 is added to detect the captured human CXCL1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

C X C motif chemokine 1, CXCL1, FSP, GRO, GRO alpha(1 73), GRO1, GROa, Growth regulated alpha protein, MGSA, MGSA a, NAP 3, SCYB1

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

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