Human CXCL1 ELISA Kit

Sensitivity

32.4 pg/mL

Range

62.5-4000 pg/mL

Reactivity

Human

Publications

6

Cat no : KE00133

Synonyms

C X C motif chemokine 1, CXCL1, FSP, GRO, GRO alpha(1 73), GRO1, GROa, Growth regulated alpha protein, MGSA, MGSA a, NAP 3, SCYB1

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Validation Data Gallery

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  Standard curve of KE00133 Human CXCL1 ELISA Kit.

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  Standard curve of KE00133 Human CXCL1 ELISA Kit.

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Product Information

KE00133 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL1 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL1. The assay recognizes human CXCL1. An antibody specific for CXCL1 has been pre-coated onto the microwells. The CXCL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CXCL1 is added to detect the captured human CXCL1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

• Product name: Human CXCL1 ELISA Kit
• Tests: 1 X 96 well plate
• Sample type: Serum, Plasma, Cell culture supernatants
• Assay type: Sandwich
• Sensitivity: 32.4 pg/mL
• Range: 62.5-4000 pg/mL
• Reactivity: Human
• Tested applications: Sandwich ELISA
• Gene ID (NCBI): 2919

Recovery

Sample Type	Average	Range
Human plasma	90%	77%-102%
cell culture supernatants	93%	78%-111%

IntraAssay

Sample	n	mean ( pg/mL)	SD	CV%
1	20	1,784.4	89.6	5.0
2	20	474.2	11.8	2.5
3	20	115.2	1.9	1.6

InterAssay

Sample	n	mean ( pg/mL)	SD	CV%
1	24	1,812.5	121.9	6.7
2	24	472.1	10.3	2.2
3	24	112.8	6.9	6.1

Background Information

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

Properties

Storage Instructions	All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms	C X C motif chemokine 1, CXCL1, FSP, GRO, GRO alpha(1 73), GRO1, GROa, Growth regulated alpha protein, MGSA, MGSA a, NAP 3, SCYB1

Publications

Species	Sample Type	Title
human	serum	Cell Rep; Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer; Authors - Xinyu Yang
human	cell culture supernatants	Int J Mol Sci; The P72R Polymorphism in R248Q/W p53 Mutants Modifies the Mutant Effect on Epithelial to Mesenchymal Transition Phenotype and Cell Invasion via CXCL1 Expression.; Authors - Cristabelle De Souza
human	cell culture supernatants	Phytother Res; Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke-induced airway inflammation.; Authors - Qing-Cuo Ren
mouse	serum	World J Gastroenterol; Calycosin attenuates severe acute pancreatitis-associated acute lung injury by curtailing high mobility group box 1 - induced inflammation; Authors - Chang-Ju Zhu
human	cell culture supernatants	Pathol Res Pract; M2 macrophages promote PD-L1 expression in triple-negative breast cancer via secreting CXCL1; Authors - Lifen Zhang
human	cell culture supernatants	Aging (Albany NY); Targeting PRKDC activates the efficacy of antitumor immunity while sensitizing to chemotherapy and targeted therapy in liver hepatocellular carcinoma; Authors - Yitong Pan