Human Carbonic Anhydrase IX/CA9 ELISA Kit
Cat no : KE00694
Synonyms
CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX
Validation Data Gallery
Product Information
KE00694 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Carbonic Anhydrase IX/CA9 ELISA kit is to be used to detect and quantify protein levels of endogenous human Carbonic Anhydrase IX/CA9. The assay recognizes human Carbonic Anhydrase IX/CA9. An antibody specific for human Carbonic Anhydrase IX/CA9 has been pre-coated onto the microwells. The human Carbonic Anhydrase IX/CA9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Carbonic Anhydrase IX/CA9 is added to detect the captured human Carbonic Anhydrase IX/CA9 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Carbonic Anhydrase IX/CA9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 2.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 768 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 84% | 72%-112% |
| Cell culture supernatant | 103% | 99%-109% |
| Urine | 109% | 103%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,291.3 | 32.3 | 2.5 |
| 2 | 8 | 313.5 | 7.9 | 2.5 |
| 3 | 8 | 143.5 | 3.4 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,241.4 | 72.6 | 5.8 |
| 2 | 16 | 300.8 | 14.9 | 4.9 |
| 3 | 16 | 148.1 | 9.2 | 6.2 |
Background Information
CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia. It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular. Under hypoxic conditions, CA9 expression increases, helping cells adapt to the acidic environment.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.