Human Caspase 1 ELISA Kit
Cat no : KE00231
Synonyms
CASP 1, CASP1, Caspase 1, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, Interleukin 1 beta convertase, P45
Validation Data Gallery
Product Information
KE00231 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Caspase 1 ELISA kit is to be used to detect and quantify protein levels of endogenous Caspase 1. The assay recognizes human Caspase 1. An antibody specific for Caspase 1 has been pre-coated onto the microwells. The Caspase 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human Caspase 1 is added to detect the captured human Caspase 1 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Caspase 1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell cuture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 834 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 84% | 77%-92% |
| Cell cuture supernatants | 100% | 92%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 197.4 | 6.2 | 3.1 |
| 2 | 20 | 46.3 | 1.5 | 3.3 |
| 3 | 20 | 10.2 | 0.7 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 198.0 | 12.1 | 6.1 |
| 2 | 24 | 50.1 | 4.2 | 8.4 |
| 3 | 24 | 11.9 | 0.9 | 7.9 |
Background Information
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products(PMID:21804020).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CASP 1, CASP1, Caspase 1, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, Interleukin 1 beta convertase, P45 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.