Human Cathepsin D ELISA Kit
Cat no : KE00421
Synonyms
Cathepsin D,CTSD,Cathepsin D heavy chain,Cathepsin
Validation Data Gallery
Product Information
KE00421 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Cathepsin D ELISA kit is to be used to detect and quantify protein levels of endogenous human Cathepsin D. The assay recognizes human Cathepsin D. An antibody specific for human Cathepsin D has been pre-coated onto the microwells. The human Cathepsin D protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Cathepsin D is added to detect the captured human Cathepsin D protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Cathepsin D ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1509 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 80%-99% |
| Cell culture supernatant | 95% | 93%-96% |
| Saliva | 98% | 96%-100% |
| Human milk | 93% | 91%-94% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 19.70 | 1.24 | 6.31 |
| 2 | 20 | 4.65 | 0.14 | 3.07 |
| 3 | 20 | 1.29 | 0.06 | 4.71 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 19.97 | 1.53 | 7.67 |
| 2 | 24 | 4.69 | 0.26 | 5.56 |
| 3 | 24 | 1.41 | 0.08 | 5.44 |
Background Information
CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family, which is synthesized in rough endoplasmic reticulum as preprocathepsin D. After removal of signal peptide, the 52 kDa procathepsin D is targeted to intracellular vesicular structures (lysosomes, endosomes, phagosomes). It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Loss of CTSD leads to lysosomal dysfunction and accumulation of different cellular proteins implicated in neurodegenerative diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cathepsin D,CTSD,Cathepsin D heavy chain,Cathepsin |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Neurospine Lysophosphatidylcholine Acyltransferase 1-Phosphatidylcholine Axis Protects Nucleus Pulposus Cells From Ferroptosis by Facilitating Lysosomal Repair via Interaction With the Endoplasmic Reticulum. |